The Science

c-active-stampThe Science

C-active Microbial Technology is a plant growth promoting technology that consists of natural plant growth promoting bacteria formulated to enhance nutrient utilisation in plants, especially nitrogen. The technology is designed to compensate up to 30kg N/Ha using foliar application as the principle means of inoculation.

Mumuni Abudulai, R. Moss, J. A. Nboyine, D. Opare‐Atakora and A. Seidu. (2013), The effects of the microbial amendments, Eco‐T (Trichoderma hazianum) and Enrich (Herbaspirillum seropedicae and Bacillus subtilis) on growth and yield of cotton in Ghana. Advanced Crop Science. 3(11): 729–735


Some of the mechanisms that are responsible for increased nitrogen efficiency by microorganisms in plants include the production of plant hormones such as auxins, cytokinins, gibberellins and ethylene, P-solubilisation, vitamin production and the fixation of nitrogen (Dobbelarere, 2003). As a direct influence observed plant growth promoting effects include increased nitrogen utilisation and improved fertiliser uptake. Economic and environmental benefits can include income from higher yields, reduced fertiliser costs and reduced emissions for the greenhouse gas, NO3-N to ground water (Kennedy, 2006).

Endophytes are microorganisms that live within plants. Endophytic bacteria such as Herbaspirillum spp and Bacillus spp, which are both present in the C-active Microbial Technology formulation, are well known for their plant growth promoting capacity. Endophytes can colonise sugarcane, rice, maize, sorghum and other cereals (James et al., 2000). Microbes similar to those in C-active Microbial Technology has been observed to increase nitrogen uptake and increase yields in a variety of crops.

Ivan R. Kennedy, A. T. M. A.choudhury and Mihaly L. Kecskes. Non-symbiotic bacterial diazotrophs in crop-farming systems: can their potential for plant growth promotion be better exploited? Soil Biology and Biochemistry Volume 36, Issue 8, August 2004, Pages 1229-1244

Game Changing Benefits

Obtaining maximum benefits on farms from diazotrophic plant growth promoting biofertilisers requires a systematic strategy designed to fully utilise all beneficial factors, allowing crop yields to be maintained or even increased while fertiliser applications are reduced (Kennedy, 2006). By taking advantage of endophytes, microorganisms are protected inside the plant and have access to improved conditions that are conducive to multiplication and plant growth promotion.


Bacterial endophytes inside of plant tissues.

C-active Microbial Technology is unique because it takes advantage of the most efficient plant microorganisms using a programmed system of delivery that improves colonisation, control and reliability in a simple to use format. By focusing on a systematic strategy from manufacture to agronomy the C-active Microbial Technology becomes a unique and complete package.

Key Technical Advantages

  • Multiple strains allows the product to be versatile in a number of crops and conditions.
    • Freeze-drying creates a highly concentrated stable powder to be shipped world-wide via airfreight in a sachet.
  • Unique packaging and mixing instruction allows ease of use and consistent dilution of the inoculation before application.
  • Foliar inoculation allows direct colonisation of an established plant and allows for flexibility of application timing.
  • As a foliar inoculant the addition of the activator allows for improved colonisation through stomatal pores on the leaf surface, allowing for improved colonisation.
  • Specific application conditions that takes into account temperature and humidity allows for the best application conditions (Delta T).
  • By taking advantage of dipslides the concentration in the spray tank can be controlled allowing for improved confidence by farmers and agronomists.
  • Specialist testing developed by thinkbio allows for improved confidence in regards to colonisation potential and which crops are more difficult to inoculate.

Unique Testing

Two separate tests have been adopted to assist thinkbio further understand the complex nature of successful inoculation in different crops

Potential Infiltration and Contact

The testing has been used to estimate the capacity for a given liquid to make contact with the lead surface and potentially absorb into the plant.  Essentially it has enables us to understand how difficult a crop is to foliar inoculate and tailor the recommended application timing and rates for various crops with the key learning being that not all plants inoculate equally.

Tracer Testing (Potential Infiltration)

Tracer observations indicate a visual estimate of the potential infiltration and colonisation of the selected bacterial strains contained within the inoculant. Tracer testing has allowed us to see and understand where specifically on the plant the inoculant can potentially absorb into the leaf which is known to vary significantly depending on the crop.